Description
Allows for fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry.
Application
Fluorescence
Excitation wavelength in the range of 450-500 nm (e.g. 488 nm) and detection in the range of 515-565 nm (green).
Flow cytometry
Analyze on a flow cytometer using 488 nm excitation and a 515 nm band pass filter for fluoresce in detection and a filter >600 nm for PI detection.
Allows for fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry.
Application
Excitation wavelength in the range of 450-500 nm (e.g. 488 nm) and detection in the range of 515-565 nm (green).
Analyze on a flow cytometer using 488 nm excitation and a 515 nm band pass filter for fluoresce in detection and a filter >600 nm for PI detection.
No. |
Component |
Amount |
Cap |
1 |
AnnexinV-FITC |
550 µl |
Green |
2 |
Propidiumiodide |
600 µl |
Red |
3 |
Binding buffer |
300 ml |
Nature |

