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Allows for fluorescent detection of Annexin V bound to apoptotic cells and quantitative determination by flow cytometry.
Description
Catalog No:AVK250
Specification :250 Assay
Shelf Life:Store at 2-8℃
Application:Early detection of Apoptosis
Analysis:
Excitation wavelength in the range of 450-500 nm (e.g. 488 nm) and detection in the range of 515-565 nm (green).
Analyze on a flow cytometer using 488 nm excitation and a 515 nm band pass filter for fluoresce in detection and a filter >600 nm for PI detection.
| No | Component | Volume | Cap Color |
| 1 | AnnexinV-FITC | 550µl | Green |
| 2 | Propidiumiodide | 600µl | Red |
| 3 | Binding buffer | 300ml | Transparent |
No.1
| Component | AnnexinV-FITC |
| Volume | 550µl |
| Cap Color | Green |
No.2
| Component | Propidiumiodide |
| Volume | 600µl |
| Cap Color | Red |
No.3
| Component | Binding buffer |
| Volume | 300ml |
| Cap Color | Transparent |